USDA 5018.00
Heartworm
Antigen Test Kit
An easy to use immunochromatographic test for the qualitative detection of Dirofilaria immitis in canine serum, plasma, or whole blood.
Available Sizes: 10 tests, 100 tests
- Remove the test device from the foil pouch, and place it on a flat and dry surface.
- Draw up the specimen using the disposable dropper.
- Add one drop (approximately 40μl) of canine serum, plasma or whole blood into the sample hole, and then add four drops (approximately 160μl) of the assay diluent as shown in the figure below. If the migration has not appeared after 1 minute, add one more drop of the assay diluent to the sample well.
- As the test begins to work, you will see a purple color move across the result window in the center of the test device.
- Interpret test results at 5-10 minutes. Do not decide after 10 minutes.
- Free confirmatory test
- Two year shelf-life
- Room temperature storage
- Highly sensitive and specific
- Fast results within 5 minutes
- Simple and easy to use
- Small sample volume (40ul)
- Highly purified antibodies produced by biotechnology for the detection and capture of heartworm antigens
Frequently Asked Questions
Yes! Please email the attached form and a photo of the test cassette with positive result to info@modernveterinarytherapeutics.com.
We recommend EDTA or heparin for whole blood collection, however you can also use citrate. If results are doubtful, please use serum.
The One-Step Heartworm Test Kit and the Heartworm Test Kit both have the same level of sensitivity and specificity, but they have distinct test procedures and include different materials. The One Step Heartworm Test Kit contains vials with EDTA, disposable droppers, and the test devices, whereas the Heartworm Test Kit includes assay diluent bottles, disposable droppers, and test devices. Because the materials vary, the test procedures for these kits are also different.
The assay diluent is specific to our test. We do not recommend the use of any other solution as we cannot guarantee that the product will work properly.
Any positive result should be confirmed prior to initiating heartworm treatment. A confirmatory test should use a different antigen detection method as the original screening test, thus we recommend a Knot test for detection of microfilariae and a well-ELISA.
A false positive (or negative) result from an individual animal not resulting from technique errors will continue to occur on multiple tests of the same type. Therefore, the false result is usually caused by some unique characteristics of the particular animal, as opposed to a defective test. This characteristic could be a component of the animal’s blood, which is able to react in a test’s system to show a positive result in the absence of heartworm antigens or a negative result in the presence of heartworm antigens.
The following situations may cause this situation:
- Animal could have adult worms that die and be left with circulating microfilariae.
- Microfilariae could be Dipetalonema reconditum, a parasite which does not produce the immitis antigen that our test detects.
- Young dogs born to heartworm-positive mothers could be infected transplacentally and be born with circulating microfilariae, but not have adult D. immitis, therefore no detectable D. immitis antigen.
- Occasionally, microfilariae will be present before detectable levels of antigen have accumulated.
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